reduction in subgingival bacterial counts (log 1.9 ± 0.7) than pos-itive control treatment (log 1.1 ± 0.6) and subgingival plaque sampling alone (log 0.5 ± 0.5; P <0.05). Differences between pos-itive and negative control were not signiﬁcant (P <0.05). Conclusion: The novel low abrasive air polishing powder is

Begränsat utrymme gör provtagning den sunda subgingival sulcus av five putative periopathogenic bacteria in subgingival plaque samples.

The tech-nique used may inﬂuence the outcome of the mi-crobiological analysis.5,6 Also, the number of sample sites is essential, especially when samples are pooled.7 Microbial subgingival sampling is time con-suming and involves the selection of sampling sites, isolation and drying of these sites, and 2015-07-21 BACKGROUND The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results comparable with standard sampling method (multiple Reproducibility of a commercially available subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes. Pretzl B, Paul J, Krigar DM, Uhlmann L, Eickholz P, Dannewitz B. Acta Odontol Scand, 75(4):302-307, 22 Mar 2017 Cited by: 1 article | PMID: 28325127 Subgingivale Plaque, Plaqueentnahme, Plaqueentnahmetechnik, Parodiagnostik, Plaque-Analyse mit Real Time PCR, Papierspitzen, subgingival plaque, plaque sampling techniques, plaque analysis with real-time PCR, paper point sampling, curette sampling 2.4. Subgingival Plaque Sampling and Quantitative Bacterial Assay. After removing the supragingival plaque, a subgingival plaque sample was taken by inserting 2 sterile number 40 paper points (Zipperer Absorbent Paper Points, VDW GmbH, Munich, Germany) consecutively into the periodontal pocket for 10 s at each of the selected sites. Plaque sampling for DNA test was performed at the baseline, 1 week after professional tooth cleaning, and after 3 weeks of either miswak or toothbrush use.

Subgingival plaque sampling

After supragingival plaque was removed with a cotton roll, subgingival plaque was 29 Nov 2014 JJose-Luis Ruiz, DDS, shows how keeping restorative margins should not be left to luck, but to a supragingival protocol to avoid them. 15 Nov 2012 Dr. Buddy Mopper demonstrates a simple technique to close a subgingival margin of a composite restoration while maintaining the integrity of 21 Oct 2014 PAG set available on http://www.dental-pag-official.com the exclusive distributor. Argumentaire scientifique et clinique Disadvantages of the technique. require training for proper collection; specific equipment and supplies required for sampling; More validation is needed. 29 Aug 2017 Patients with angiographic coronary artery disease with elevated low-density lipoprotein (LDL) cholesterol on chronic statin therapy were 28 Aug 2014 Ladders allow you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected band sizes.

Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth. To evaluate the influence of sampling on the microflora, plaque samples were also taken twice at 2 teeth without therapy with PD of 3 to 5 mm (negative control).

However, the collection of saliva 22 Mar 2017 Material and methods: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens. Blood and pus at the gingivalis in saliva and in subgingival plaque samples, showing comparable specificity to culture and real-time PCR. Conclusion: We applied the FRET technology 2 Jan 2018 DNA was then extracted from plaque samples and analyzed by 16S.

av T Honnér — of this study was to find a sampling technique to investigate the relationship between proteins and bacteria in subgingival biofilms. Method: Three membranes

Choose your ladder based on the expected band sizes. Always run leukocytes associated with the subgingival dental plaque samples. These Findings are related to previous bacteriologic studies of ]P dental plaque. tively characterize the total culturable subgingival-plaque flora of one dog with ligatures and one dog without.

Aggregatibacter actinomycetemcomitans had been detected subgingivally in all prior to anti-infective therapy (AT) and METHODS: In 100 patients with aggressive/chronic periodontitis, subgingival plaque was sampled from the deepest pockets per quadrant (MT4) and per sextant (MT6). Plaque samples were taken using two sterile paper points simultaneously. One paper point from each pocket was pooled with the three other paper points of the pockets (MT4). Two Subgingival Plaque‐Sampling Strategies Used With RNA Probes Diana‐Maria Krigar Section of Periodontology, Department of Conservative Dentistry, Clinic for Oral, Dental, and Maxillofacial Diseases, University Hospital Heidelberg, Heidelberg, Germany. the subgingival microbiota, pathogens, and plaque [12]. In the present study, a gingival retraction cord (a specific dentistry tool) was used to sampling GCF and plaque: this is a novel sampling method that is potentially more reproducible and less technique-sensitive than the paper point sampling method. Each period consisted of an initial professional dental cleaning procedure done at the university clinic, then a 3 week plaque accumulation period, followed by a return to the clinic for supra- and subgingival plaque sampling.
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subgingival plaque; periodontal pathogens;. Subgingival plaque sampling and scaling and root planing were performed two weeks after clinical data recording. Two weeks later irrigation procedures were 7 Feb 2017 The hypervariable region V4 of the 16S rDNA gene was targeted to explore richness and diversity of subgingival plaque samples [11].
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Reproducibility of subgingival bacterial samples from patients with Effect of oxybenzone on PGE2-production in vitro and on plaque and gingivitis in vivo.

Methods: Subgingival plaque of rheumatoid arthritis patients with periodontitis (N=66) or gingivitis (N=15) was collected at two timepoints by sterile curettes and paperpoints. Subgingival plaque was collected from a total of 90 pockets across all subjects. Subsequently, subgingival ultrasonic instrumentation (SUI) was performed by using EO or saline as the irrigation agent.

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Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens. Blood and pus at the

Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Of the sampling of all assessed sites, the highest probability for simultaneously detecting the tested pathogens was found in respect to the combination of supra- and subgingival plaque samples 1994-08-01 Conclusion: Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP. Discover the world's research. Subgingival plaque sampling and clinical recording (at baseline) and scaling and root planing was performed. Two weeks later the selected periodontal sites were submitted to one of the following treatments: Irrigation with a hydroalcoholic solution of propolis extract twice a week for 2 weeks (group A); irrigation with a placebo twice a week for 2 weeks (group B); or no additional treatment Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth.

In this investigation, we determined whether the association between BANA hydrolysis and spirochetes could be obtained in pooled subgingival plaque samples

Strand P, Palmer RM, Wilson RF. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Methods: Samples were taken from the following intraoral sites in 35 patients with untreated chronic periodontitis before and 1.5, 3, and 6 months after non-surgical periodontal therapy: supra- and subgingival plaque from the deepest pockets in each sextant; pooled supra- and subgingival plaque from another six randomly selected, less affected teeth; mucosal swab samples from the tongue, tonsils, throat, and buccal mucosa; and stimulated and unstimulated saliva. Microbial species were A subgingival plaque sample was then obtained using a Gracey curette machined to a blade width of 0.5 mm to facilitate access to the subgingal area.

Two sterile paper points were inserted simultaneously into the periodontal pockets. 1. Oral Microbiol Immunol. 1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease.